Fig. 2 | Scientific Reports

Fig. 2

From: Handheld multiphoton and pinhole-free reflectance confocal microscopy enables noninvasive, real-time cross-sectional imaging in skin

Fig. 2

Simultaneous multimodal, four-channel imaging with cellular resolution. (a) An in vivo, four-channel capture of normal human skin (Fitzpatrick skin type [FST] 4) with each channel shown individually as a grayscale image: (a) 2PS, (b) 2PL, (c) RCM, (d) SHG, and (e) composite. All four channels are collected simultaneously in vivo for registered features (f–i). Zoom of the dash-bounded box: (f) 2PS, (g) 2PL, (h) RCM, and (i) SHG. (a–i) Scale bars, 100 µm. FOV setting; standard. (j) In vivo, cross section detail of summed 2PS and 2PL signal in the basal layer of the epidermis. Intercellular spaces and filamentous structures (white arrows) within the epidermis are smaller than 2 µm diameter white circles. FOV setting; zoom. (k) Representative image of a 0.5 µm fluorescent bead. Scale bar, 15 µm. FOV setting; zoom. (l) Representative intensity plot through bead axis used to calculate system resolution.

Back to article page