Fig. 2

Representative images from embryo relocation surgical procedure and follow-up. Images (A-I) are shown with superior (towards the head) aspect at the bottom left corner and inferior (towards the tail) aspect towards the top right corner. (A) Visualization of uterine horns containing gestational sacs (thick black arrows). (B) Insertion of first suture, superior to the gestational sacs (thick green arrows) to be relocated. (C) Ligated portion of the uterine horn to be excised. (D) Excision of the ligated portion of the uterine horn. (E) Removal of mesometrial fat on remaining portion of the uterine horn containing gestational sacs to be relocated. (F) Portion of the uterine horn containing gestational sacs to be relocated prior to relocation to other horn. (G) Creation of the flap in the receiving uterine horn. Inset indicates the flap once created. (H) Gestational sacs being relocated onto flap. (I) Relocated gestational sacs fully enclosed by flap. (J) Rat in the Embryo Relocation group at full-term (D20/D21), prior to tissue collection. Visualization of uterus surrounding the relocated fetus and mesometrial blood vessels. (K) Representative ultrasound image of fetus from full-term rat in Embryo Relocation group, with the head, spine and rib cage labelled.