Fig. 1
From: Acute hyperlipidemia has transient effects on large-scale bone regeneration in male mice
Schematic timeline of the mouse experimental setup. Young C57BL/6J mice (4–5 weeks old) from each strain (wild-type/WT and deficient for the low-density lipoprotein (LDL) receptor gene/Ldlr−/−) and sex were randomly assigned to different dietary groups 10 days before undergoing rib resection surgery (baseline). Animals were fed a low-fat diet (LFD) or high-fat diet (HFD) for a total of 31 days. Rib resection surgeries were performed 10 days after exposure to LFD or HFD (0 dpr) at 6–7 weeks of age and mice were monitored for any signs of bone regeneration by using live micro-computed tomography (µCT) data analysis at 2, 7, 14 and 21 days post-resection (dpr). At 21 dpr, all mice were euthanized, and ribs were dissected for subsequent analysis of ex vivo high resolution µCT data. The ruler shown at 0 dpr illustrates the length of the rib that is removed (3 mm) in each surgical procedure.
