Fig. 3
Comparative assessment of presenilin-associated protein (PSAP) and phosphorylated tau (pTau) immunolabeling in paraffin sections and their protein elevations in pAD/AD human cortical lysates. (A-A4, B-B4) Micrograph panels show PSAP (A-A4) and pTau (B-B4) immunolabeling with hematoxylin counterstain in adjacent temporal lobe sections from an AD case. PSAP and pTau labeled somata (arrows) and neurites (hallowed arrows) show similar morphological features. The nuclei in the somata with heavy tangle-like products appear to be lost, dislocated near the cell border, or shrunken (A1-A4, B1-B4). PSAP-labeled neurites are mostly elongated, while pTau-labeled neurites appear elongated (hallowed arrows) as well as globular (arrowheads). (C) show the immunoblotted PSAP (assayed with 10% SDS-PAGE gel) and pTau (AT8, assayed with 10% SDS-PAGE gel). (D) show the bands of total tau (TAU-5, assayed with 12% SDS-PAGE), and Aβ products blotted with the antibody D12B2 (assayed with 15% SDS-PAGE gel. (E) The full-length protein and clearage fragment of the β-amyloid precursor protein (APP) and Aβ productions blotted with the 6E10 antibody (assayed 12% SDS-PAGE gel). Immunoblots from a set of cortical lysates from four pAD/AD cases and four neuropathology-free controls (CTL) are presented as indicated. Original western blot images were shown in Additional file 1. (F) The bar/dot graphs show the densitometric data and statistics from the two groups as indicated (mean ± SD, n = 8/group, two-tailed unpaired t-test). Arrowheads point to the putative monomer Aβ productions. CA1-CA3: Ammon’s horn subregions; DG: dentate gyrus; FG: fusiform gyrus; GCL: granule cell layer: Hi: hilus; PHG: parahippocampal gyrus; Pro-S: prosubiculum; Pre-S: presubiculum; Sub: subiculum; s.p.: stratum pyramidale; s.r.: stratum radiatum, WM: white matter.
