Fig. 5

Triple fluorescent characterization of presenilin-associated protein (PSAP), phosphorylated tau (pTau) and Amylo-Glo labeling in pAD/AD human brain sections. (A) The low power view of triple-labeled temporal lobe paraffin section, with framed areas enlarged. (B) Neuritic plaques (hallowed triangles) in the molecular layer of the dentate gyrus. A great majority of the dystrophic neurites appear triple-labeled, with the Amylo-Glo also staining extracellular amyloid deposits. (C, D) Varied colocalization patterns of PSAP, pTau and Amylo-Glo among somal profiles, which can be identified as five groups: PSAP/AT8/Amylo-Glo triple-labeled (pointed by white arrows), PSAP/Amylo-Glo double-labeled (pointed by white arrowheads), AT8/Amylo-Glo double- labeled (hallowed white arrows), Amylo-Glo single labeled (hollowed arrowheads) and AT8 single labeled (two-tailed arrows). (D) The numbers of the five types of neuronal somata were quantified in four brains, with the calculated cell type ratios indicated (mean ± SD, n = 4, one-way ANOVA together with Bonferroni post hoc test). CA1-CA3: Ammon’s horn subregions; DG: dentate gyrus; Ent: entorhinal cortex; GCL: granule cell layer; Hi: hilus; ML: molecular layer; PHG: parahippocampal gyrus; Pro-S: prosubiculum; Pre-S: presubiculum; Sub: subiculum. Antibodies and fluorescence channels, and scale bars, are as indicated in the image panels.