Fig. 7 | Scientific Reports

Fig. 7

From: Increased expression of the proapoptotic presenilin associated protein is involved in neuronal tangle formation in human brain

Fig. 7

Double immunofluorescent characterization of presenilin-associated protein (PSAP) and sortilin in pAD/AD human brains. (A) Section map, with enlarged areas show that the majority of PSAP-enriched neuronal somata (pointed by arrows) are not colocalized with sortilin immunoreactivity across the regions (B-E), while a few of cells colocalized somata observed (pointed by arrowhead). No sortilin colocalization is seen in PSAP-labeled dystrophic neurites (C, open arrows). The DAPI nuclear stain in the PSAP enriched neurons appears reduced or lost. (F) The numbers of PSAP-positive neurons with and without sortilin labeling counted in the parahippocampal neocortex and subicular to CA1 pyramidal layers, with the ratios calculated (mean ± SD, n = 4, two-tailed unpaired t-test). CA1-CA3: Ammon’s horn subregions; DG: dentate gyrus; Ent: entorhinal cortex; PHG: parahippocampal gyrus; Pro-S: prosubiculum; Pre-S: presubiculum; Sub: subiculum. Antibodies and fluorescence channels, and scale bars, are as indicated in the image panels.

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