Figure 6
Antibacterial and antibiofilm abilities of magnetron sputtering silver coating. (A) Colony count after incubation with Staphylococcus aureus for 24 h by co-culture method and membrane contact method. ab: magnetron sputtering silver coated titanium alloy group, cd: uncoated titanium alloy group, ac: co-culture method, bd: membrane contact method. (B) Time / CFU curve of the silver coated and uncoated groups after incubation with Staphylococcus aureus over time by co-culture method. (C) Bacteria live and dead staining of Staphylococcus aureus after cultured with silver coated titanium alloy for 24 h by co-cultured method or membrane contact method. As shown, there was almost no green stained live bacteria in the membrane contact method group while there were full of green stained live bacteria in the co-culture method group, which further supported the excellent contact antibacterial feature of the magnetron sputtering silver coating. (D) Representative SEM images of Magnetron sputtering silver coated titanium alloy (a) and uncoated titanium alloy (b). No obvious biofilm formation was observed on the surface of silver coated titanium alloy while there were large areas of biofilms on the surface of uncoated titanium alloy.
