Table 5 The changes of amino acid content with aliphatic, hydroxyl and amide groups in tobacco leaves under different curing time.

From: Regression prediction of tobacco chemical components during curing based on color quantification and machine learning

Location	Curing time (h)	Indicators (mg/g)
		Aliphatic amino acid content				Amino acid content with hydroxyl and amide groups
		Glycine	Isoleucine	Leucine	Valine	Threonine	Serine	Asparagine
Upper leaves	0	0.03 ± 0^c	0.09 ± 0.01^abc	0.18 ± 0.02^a	0.17 ± 0.03^d	0.21 ± 0.03^a	0.28 ± 0.06^a	0.37 ± 0.22^d
	24	0.03 ± 0.01^c	0.07 ± 0.02^bc	0.13 ± 0.03^bc	0.21 ± 0.05^cd	0.24 ± 0.06^a	0.30 ± 0.04^a	0.97 ± 0.07^cd
	48	0.04 ± 0.01^bc	0.07 ± 0.01^bc	0.15 ± 0.02^abc	0.18 ± 0.04^d	0.20 ± 0.02^ab	0.21 ± 0.03^b	2.47 ± 0.58^ab
	60	0.04 ± 0.01^bc	0.07 ± 0^c	0.13 ± 0.01^bc	0.17 ± 0.03^d	0.15 ± 0.03^bc	0.16 ± 0.03^bc	2.87 ± 0.41^a
	72	0.05 ± 0^abc	0.07 ± 0.01^c	0.12 ± 0.02^bc	0.16 ± 0.02^d	0.10 ± 0.03^cd	0.11 ± 0.03^cd	2.06 ± 0.58^ab
	84	0.06 ± 0.01^ab	0.07 ± 0.01^c	0.11 ± 0.01^c	0.17 ± 0.03^d	0.08 ± 0.02^d	0.10 ± 0.02^d	2.13 ± 0.58^ab
	96	0.06 ± 0.01^ab	0.07 ± 0^bc	0.10 ± 0.01^c	0.19 ± 0.03^cd	0.07 ± 0.01^d	0.08 ± 0.02^d	1.92 ± 0.41^b
	108	0.06 ± 0^ab	0.09 ± 0.02^abc	0.11 ± 0.02^c	0.26 ± 0.05^c	0.07 ± 0.01^d	0.08 ± 0.01^d	1.86 ± 0.67^b
	120	0.05 ± 0^abc	0.11 ± 0.01^ab	0.12 ± 0.01^c	0.34 ± 0.06^b	0.07 ± 0.01^d	0.08 ± 0.01^d	1.72 ± 0.48^bc
	132	0.07 ± 0.04^a	0.12 ± 0.04^a	0.16 ± 0.04^ab	0.40 ± 0.04^a	0.08 ± 0.01^d	0.08 ± 0.01^d	1.72 ± 0.40^bc
Middle leaves	0	0.02 ± 0^d	0.06 ± 0.02^c	0.11 ± 0.02^abc	0.09 ± 0.01^d	0.13 ± 0.01^a	0.20 ± 0.02^b	0.14 ± 0.02^d
	24	0.03 ± 0^cd	0.06 ± 0.01^c	0.10 ± 0.01^abc	0.14 ± 0.01^cd	0.12 ± 0.02^ab	0.27 ± 0.03^a	0.77 ± 0.08^cd
	48	0.03 ± 0.01^bc	0.06 ± 0.01^c	0.11 ± 0.02^abc	0.13 ± 0.01^d	0.08 ± 0.01^cd	0.16 ± 0.03^b	2.13 ± 0.75^bcd
	60	0.04 ± 0^a	0.06 ± 0.01^c	0.12 ± 0.02^ab	0.16 ± 0.02^cd	0.09 ± 0.03^bc	0.18 ± 0.07^b	3.80 ± 0.46^ab
	72	0.04 ± 0.01^a	0.06 ± 0^c	0.12 ± 0.02^abc	0.15 ± 0.01^cd	0.08 ± 0.03^cde	0.15 ± 0.06^bc	4.57 ± 0.43^a
	84	0.04 ± 0^a	0.05 ± 0.01^c	0.09 ± 0.01^bc	0.14 ± 0.04^cd	0.05 ± 0^e	0.09 ± 0.01^cd	2.96 ± 1.01^abc
	96	0.04 ± 0^a	0.06 ± 0^c	0.09 ± 0.01^c	0.16 ± 0.01^cd	0.06 ± 0.01^de	0.09 ± 0.03^cd	3.06 ± 0.68^abc
	108	0.04 ± 0^a	0.09 ± 0.03^bc	0.11 ± 0.02^abc	0.25 ± 0.10^bc	0.06 ± 0.01^de	0.09 ± 0.01^cd	2.98 ± 1.26^abc
	120	0.04 ± 0^a	0.12 ± 0.03^ab	0.13 ± 0.02^a	0.32 ± 0.08^ab	0.07 ± 0.01^cde	0.09 ± 0.01^cd	2.69 ± 0.91^abc
	132	0.04 ± 0^ab	0.14 ± 0.04^a	0.11 ± 0.03	0.39 ± 0.11^a	0.08 ± 0.01^cde	0.08 ± 0.02^d	2.37 ± 0.85^abcd

Note: Different letters indicate significant differences (P ≤ 0.05) at different curing times for the same part of the tobacco.

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Table 5 The changes of amino acid content with aliphatic, hydroxyl and amide groups in tobacco leaves under different curing time.

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