Table 6 The changes of amino acid content with sulfur, subamino, non-protein and total in tobacco leaves under different curing time.

From: Regression prediction of tobacco chemical components during curing based on color quantification and machine learning

Location	Curing time (h)	Indicators (mg/g)
		Sulfur, subamino acid content		Non-protein amino acid content			Total free amino acids
		Cystine	Proline	β-alanine	β-Aminoisobutyric	γ-aminobutyric	Total free amino acids
Upper leaves	0	0.10 ± 0.02^c	0.15 ± 0.08^e	0.16 ± 0.08^d	0.11 ± 0.02^a	1.10 ± 0.19^a	3.68 ± 0.44^c
	24	0.11 ± 0.02^c	1.40 ± 0.59^e	0.27 ± 0.06^bc	0.08 ± 0.01^ab	0.83 ± 0.18^b	5.44 ± 0.13^c
	48	0.12 ± 0.01^bc	5.45 ± 0.92^d	0.18 ± 0.07^cd	0.07 ± 0^b	0.73 ± 0.1^bc	10.12 ± 1.69^b
	60	0.13 ± 0.01^abc	7.82 ± 0.60^c	0.18 ± 0.04^cd	0.08 ± 0^ab	0.79 ± 0.03^bc	15.14 ± 1.85^a
	72	0.11 ± 0.02^bc	8.60 ± 0.85^bc	0.15 ± 0^d	0.07 ± 0.01^b	0.86 ± 0.11^b	17.02 ± 1.39^a
	84	0.12 ± 0.02^bc	9.88 ± 0.81^ab	0.17 ± 0.01^cd	0.07 ± 0.01^b	0.78 ± 0.11^bc	15.79 ± 0.98^a
	96	0.13 ± 0.01^abc	10.66 ± 0.23^a	0.22 ± 0.04^cd	0.06 ± 0.01^b	0.69 ± 0.1^bcd	15.55 ± 1.50^a
	108	0.13 ± 0.01^abc	8.98 ± 0.69^abc	0.24 ± 0.02^bcd	0.07 ± 0.01^b	0.62 ± 0.06^bcd	15.68 ± 1.51^a
	120	0.18 ± 0.03^a	8.92 ± 0.71^abc	0.36 ± 0.06^a	0.08 ± 0.04^b	0.55 ± 0.02^cd	15.33 ± 0.87^a
	132	0.17 ± 0.05^ab	9.39 ± 0.76^abc	0.33 ± 0.05^ab	0.07 ± 0.03^b	0.46 ± 0.05^d	14.21 ± 1.85^a
Middle leaves	0	0.05 ± 0.01^c	0.07 ± 0.02^c	0.17 ± 0.08^cde	0.07 ± 0.04^ab	1.07 ± 0.09^a	4.97 ± 0.68^c
	24	0.06 ± 0^c	1.00 ± 0.22^c	0.23 ± 0.05^bc	0.08 ± 0.01^a	0.82 ± 0.08^b	6.86 ± 0.20^c
	48	0.06 ± 0^bc	4.27 ± 0.66^b	0.16 ± 0.07^cde	0.06 ± 0.02^abcd	0.70 ± 0.09^bc	12.82 ± 1.09^b
	60	0.07 ± 0.01^bc	6.91 ± 0.30^a	0.09 ± 0.03^e	0.07 ± 0.02^abc	0.80 ± 0.05^b	16.11 ± 1.27^a
	72	0.07 ± 0.01^abc	7.89 ± 1.07^a	0.13 ± 0.01^de	0.05 ± 0^bcd	0.69 ± 0.06^bc	16.00 ± 1.95^a
	84	0.06 ± 0^bc	8.61 ± 0.17^a	0.14 ± 0.02^de	0.04 ± 0^d	0.65 ± 0.08^c	17.45 ± 1.25^a
	96	0.07 ± 0.01^bc	8.35 ± 1.32^a	0.20 ± 0.02^cd	0.04 ± 0^cd	0.60 ± 0.07^cd	17.71 ± 0.61^a
	108	0.07 ± 0.01^abc	8.53 ± 0.54^a	0.28 ± 0.03^b	0.05 ± 0.02^bcd	0.57 ± 0.07^cd	15.72 ± 1.68^ab
	120	0.09 ± 0.01^ab	8.35 ± 0.30^a	0.38 ± 0.05^a	0.08 ± 0.01^ab	0.49 ± 0.07^de	15.53 ± 1.59^ab
	132	0.09 ± 0.02^a	7.83 ± 1.46^a	0.41 ± 0.05^a	0.08 ± 0.01^ab	0.38 ± 0.11^e	15.60 ± 1.21^ab

Note: Different letters indicate significant differences (P ≤ 0.05) at different curing times for the same part of the tobacco.

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Table 6 The changes of amino acid content with sulfur, subamino, non-protein and total in tobacco leaves under different curing time.

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