Fig. 6 | Scientific Reports

Fig. 6

From: ERα status of invasive ductal breast carcinoma as a result of regulatory interactions between lysine deacetylases KAT6A and KAT6B

Fig. 6

Downregulation of KAT6A/B mRNAs and protein expression by siRNA-induced knock-down in breast cancer cell lines MCF-7 and MDA-MB-231. Quantitative polymerase chain reaction was used to analyze KAT6A, KAT6B and ERα mRNA expression in MCF-7 cell line (A) and KAT6A and KAT6B in MDA-MB-231 cell line (B). Western blot analysis (F) measured the effects of siRNA-mediated knockdown of KAT6A, KAT6B and ER in MCF-7 (C) and KAT6A/B in MDA-MB-231 (D) cell lines. GAPDH was used as an internal control (E). Data represent the mean and standard deviation of three independent experiments. Comparisons between groups were done using Student’s t-test: *p < 0.1; **p < 0.01; ***p < 0.001; ****p < 0.0001. Source data are provided as a Supplementary S1.

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