Fig. 1
EVsHSHS enhance the proliferation, migration, and tube formation of BMECs following OGD injury. (A) TEM was used to observe the morphology of EVs, and WB detected the marker proteins CD9, CD63, and TSG101 in EVs and the remaining culture supernatant. Original blots are shown in Supplementary Figures. (B) NTA measured the particle number and concentration of EVsNC and EVsHSHS. The effects of EVsHSHS on BMEC proliferation (C, n = 6), migration (D,E, n = 3), and tube formation (F,G, n = 3) after OGD are presented. Scale bar = 100 μm. CON + PBS, normoxia + PBS; OGD + PBS, hypoxia + PBS; OGD + EVsNC, hypoxia + EVs cultured with FBS; OGD + EVsBlank, hypoxia + EVs cultured with blank-serum; OGD + EVsHSHS, hypoxia + EVs cultured with HSHS-serum. Data are presented as mean ± SD. ###P < 0.001, vs. the CON + PBS group. ***P < 0.001, vs. the OGD + PBS group. ∆P < 0.05, ∆∆P < 0.01, ∆∆∆P < 0.001, vs. the OGD + EVsHSHS group. n.s., no significance.
