Fig. 2
EVsHSHS modulate the miR-126 and PIK3R2/PI3K/AKT pathway in BMECs. (A) Dual-luciferase reporter assay assessing the interaction between miR-126 and PIK3R2. (B) Quantitative real-time PCR analysis of miR-126 expression levels in BMECs. (C–F) Representative bands and histograms of key molecules in the PIK3R2/PI3K/AKT pathway. Original blots are shown in Supplementary Figures. CON + PBS, normoxia + PBS; OGD + PBS, hypoxia + PBS; OGD + EVsNC, hypoxia + EVs cultured with FBS; OGD + EVsBlank, hypoxia + EVs cultured with blank-serum; OGD + EVsHSHS, hypoxia + EVs cultured with HSHS-serum. Data are presented as mean ± SD, n = 3–4. •••P < 0.001, vs. the NC mimics + r-PIK3R2-3UTR-wt group. #P < 0.05, ###P < 0.001, vs. the Sham group. *P < 0.05, **P < 0.01, ***P < 0.001, vs. the Model group. ∆P < 0.05, ∆∆P < 0.01, ∆∆∆P < 0.001, vs. the EVsHSHS-H group. n.s., no significance.
