Fig. 2
From: Dynamic reorganization of multivesicular bodies and exosome production impacted by sonoporation
Sonoporation-induced dynamic changes in RAW 264.7 cells. (a) A bright field (BF) image (left) and a PI image overlaid on the BF image before sonoporation. (b) Fluorescence images of CD63-GFP of two RAW 264.7 cells at 10s, 15s, 25s, 30s, and 40 s. Sonoporation was generated by applying an ultrasound pulse at 10 + s. White lines in each panel represent the outline of the original cell area before sonoporation and red square is the selected region. The region of interest (ROI) is defined as the region within the red square and outside the white line in each panel. (c) BF and PI images of the cells in (a) 20 min after sonoporation. (d) The CD63-GFP fluorescence intensity within the ROI vs. time. “US” indicates the time point of the ultrasound application. The vertical red dash line indicates the time point when the CD63-GFP signal within the ROI increased by 5% of the background level.
