Fig. 3
From: Dynamic reorganization of multivesicular bodies and exosome production impacted by sonoporation
Cellular responses to sonoporation of an adjacent cell. (a) A bright field (BF) (left) and overly of BF and PI image (right) of two RAW 264.7 cells, cell A and cell B, before sonoporation. (b) Fluorescence images of CD63-GFP of the RAW 264.7 cells in (a) at different time points after application of an ultrasound pulse at 10 s. White lines outline the original area of cell A before ultrasound application at 10 s. Red line in each panel encloses the selected region of interest (ROI). The yellow arrow in each panel indicates a protrusion with increased CD63-GFP signals from cell A after ultrasound application. (c) BF and PI images of the two cells in (a) 20 min after ultrasound application. The PI signal (red) indicate a non-sonoporated cell (cell A) adjacent to a sonoporated cell (cell B). (d) The CD63-GFP fluorescence signal intensity in the ROI in (b) vs. time. “US” indicates the time point when an ultrasound pulse was applied at 10 s. The vertical red dash line indicates the time point when the CD63-GFP signal within the ROI increased by 5% of the background level. The shaded area indicates the time duration with elevated CD63-GFP signal intensity in the ROI.
