Fig. 2
Viral titre and tropism do not account for regionally distinct neuronal transduction (A) Mice were injected with AAV-hGFAP-Cre on day 0 and sacrificed on day 21 with different viral titres (1 × 109–1 × 1013 GC/mL; designated 9–13 on graph). The average number of tdTom + cells per section in the MC and mPFC was quantified. (B) The percentage of NeuN + tdTom + /tdTom + cells in the MC (1 × 1011 GC/mL: n = 4 mice, 1 × 1012 GC/mL: n = 6 mice, 1 × 1013 GC/mL: n = 3 mice) and mPFC (1 × 1011 GC/mL: n = 4, mice, 1 × 1012 GC/mL: n = 4 mice, 1 × 1013 GC/mL: n = 7 mice) of mice that received 1 × 1011–1 × 1013 GC/mL doses of AAV-hGFAP-Cre. One-way ANOVA followed by Tukey’s multiple comparisons tests were used to assess differences between the groups. (C, D) Images of AAV5-CAG-Cre injected MC (C) and mPFC (D) at day 21. Arrowheads indicate transduced neurons (tdTom + (red), NeuN + (green), and DAPI + (blue)). Boxes are enlarged images of selected cells within the composite image. (E, F) At 21 days post-injection there is no significant difference between the average number of tdTom + cells per section in the MC and mPFC (E) or the percentage of NeuN + tdTom + cells between regions (F). Unpaired t tests were used to assess the differences between the groups in (E) and (F) (n = 5 mice per group). Data represents mean ± SEM.
