Fig. 2 | Scientific Reports

Fig. 2

From: Intrinsically disordered regions and RNA binding domains contribute to protein enrichment in biomolecular condensates in Xenopus oocytes

Fig. 2

hnRNPAB X2 enriches in L-bodies through its RBD and IDR. (a) Schematics of domain constructs. (b–e) Stage II oocytes expressing (b) mCh-hnRNPAB X2, (c) mCh-RBD, (d) mCh-IDR, or (e) free mCh (green; detected by anti-RFP IF) were co-microinjected with Cy5 LE RNA (magenta, b′-e′) to label L-bodies. Colocalization (white) is shown in the merged confocal images (b′-e′). Scale bars = 100 μm. (f) L-body enrichment was quantitated by measuring the fraction of total protein fluorescence localized to the vegetal cortical region. Shown are relative levels of L-body enrichment for hnRNPAB X2 (green, set to 1.0 ± 0.075), RBD (blue, 0.71 ± 0.076), IDR (orange, 0.75 ± 0.059), and mCherry (red, 0.38 ± 0.034). n = 24 oocytes from four biological replicates; error bars represent standard error of the mean, **** indicates p < 0.0001, *** indicates p < 0.001, ** indicates p < 0.01, * indicates p < 0.05, and ns indicates not significant (p > 0.05). Statistics shown are an ordinary one-way ANOVA followed by Tukey’s multiple comparisons.

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