Fig. 3
(a) Purification process flowchart (partly created with BioRender.com). The phage lysate undergoes a frontal filtration, forming a production intermediate (PI), which is then tenfold diluted in PBS, forming a diluted PI (dPI), which enters tangential flow filtration (TFF) where the phage suspension is washed (wPI), then 10-time concentrated (cPI) and finally formulated (fPI) in PBS. (b) Particle concentration (yi) as a function of phage infectious titre (xi) during three replicates of purifications: (o) production intermediate, PI; (◇) diluted PI, dPI; (□) washed PI, wPI; (Δ) concentrated PI, cPI; (x) formulated PI, fPI. Each point is the mean value of three experimental measurements ± standard deviation. The dashed line represents the linear regression fitted to the experimental data set (overall N = 15). Statistical analyses: Levene’s test (p = 0.08), paired t-test (t0.05,14 = 2.15), hypothesis test for the slope (tβ1 = 23.74), and hypothesis test for the intercept (tβ0 = 0.29).
