Table 1 Regression and analytical parameters of thin layer chromatographic method for the determination of the studied components.

Calibration range (µg/band)

0.06-1

0.15-5

0.3-9

0.5–10

1-100

Slope

8268.20

664.22

-339.8^a

125.91^a

-0.2944^a

6108.6^b

2805^b

98.667^b

Intercept

665.78

158.78

1012.30

1267.30

234.01

Correlation coefficient (r)

0.9999

1

0.9999

0.9997

0.9997

Accuracy (mean ± RSD %)

100.20 ± 0.876

100.53 ± 0.709

100.06 ± 0.920

100.07 ± 1.414

98.02 ± 2.008

Precision (RSD%)**

*Repeatability

1.525

1.782

0.912

1.1039

1.276

*Intermediate precision

1.940

1.886

1.215

1.783

1.324

Robustness (%RSD)***

Ethyl acetate (8 ± 0.1 mL)

1.447

1.136

0.970

1.525

1.840

Methanol (2 ± 0.05 mL)

1.048

1.567

0.920

0.570

1.620

N-hexane (3 ± 0.05 mL)

0.870

1.180

1.435

1.210

2.008

Wavelength (245 ± 1 nm)

1.135

0.709

1.285

1.414

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