Table 4 Statistical comparison of the results obtained by the developed TLC densitometric method and the reported methods for determination of ergotamine, phenobarbital, caffeine, dipyrone and meprobamate.

From: TLC densitometric approach for concurrent determination of quinary mixture for treatment of migraine with appraisal to method greenness and whiteness

 

Proposed TLC method

Reported method

ERG

PHEN

CAF

DIP

MEP

ERG17b

PHEN24c

CAF17b

DIP24c

MEP38d

Accuracy

10.20

100.53

100.06

100.07

98.90

100.55

99.29

100.07

100.66

100.00

SD

0.876

0.709

0.920

1.414

0.566

0.962

1.514

1.053

0.577

0.995

Variance

0.767

0.503

0.846

1.999

0.320s

0.925

2.291

1.109

0.333

0.990

n

7

7

7

7

7

8

4

7

3

5

Student s t-test

0.766 (2.160)a

1.544 (2.262)a

0.019 (2.179)a

0.936 (2.306)a

2.227 (2.228)a

     

F-test

1.098 (4.210)a

4.555 (4.760)a

1.311 (4.280)a

6.003 (19.330)a

3.094 (4.530) a

     
  1. a. The values between parenthesis are corresponding to the theoretical values of t and F (p = 0.05).
  2. b. Reported RP-HPLC method for ergotamine and caffeine using Zobrax C18 (150 mm x 2.1 mm) column and gradient elusion was used with a mobile phase with consisting of water (adjusted to pH 3 using orthophosphoric acid): acetonitrile (80:20, v/v) for the first 3 min, (50:50, v/v) for the next 4.5 min, then (80:20, v/v) for the last 2.5 min. Flow rate was 0.7 mL/ min. and UV detection was at 230 nm.
  3. c. Reported RP-HPLC for dipyrone using C18 column and a mobile phase consisting of methanol-water (38, 32, v/v) set at a flow rate of 0.7 mL/min. UV detection was at 254 nm. Whilst for phenobarbital C18 column was used with mobile phase of water-ammonia-methanol (94.5:0.5:5, by volume) and detection was done at 240 nm.
  4. d. Colorimetric determination of meprobamate at 565 nm after its reaction with p-dimethylaminibenzaldehyde.
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