Fig. 1

Assessment of apoptosis induction in stem cells from human exfoliated deciduous teeth (SHED). The morphology of normal SHED (A) and apoptotic SHED (B) observed under an inverted microscope. Scale bar = 50 μm. Gel electrophoresis for polymerase chain reaction (PCR) amplification of CASP3 and CASP9 in normal and apoptosis-induced SHED (C). Annexin V & Dead Cell Assay to evaluate apoptosis in the SHED (D). Stem cell markers (CD44, CD 73, and CD90) identified via flow cytometry (E).