Fig. 7
Sodium butyrate increases H3 histone acetylation levels levels in the striatum and prefrontal cortex. Male UChB rats, voluntarily consuming a 10% ethanol solution for 44 days, were treated with either 300 mg/kg sodium butyrate or vehicle by oral gavage twice-daily for five days. An additional group of untreated animals drinking only water (naïve) was used as a control. (A) Representative Western Blot showing striatal levels of H3 histone acetylation (AcH3). Histone H4 and β-actin were used as housekeeping genes. (B) Quantification of striatal AcH3 levels normalized to the nuclear marker histone H4. (C) Quantification of striatal AcH3 levels normalized to the housekeeping gene β-actin. (D) Representative Western Blot showing prefrontal cortex levels of H3 histone acetylation (AcH3). (E) Quantification of prefrontal cortex AcH3 levels normalized to the nuclear marker histone H4. (F) Quantification of prefrontal cortex AcH3 levels normalized to β-actin. Data are presented as mean ± SEM. N = 6 animals per group. *p < 0.05, **p < 0.01. One-way ANOVA followed by Bonferroni post-hoc test.
