Table 4 Antioxidant activity of the samples by β-Carotene-linoleic acid, DPPH•, ABTS•+, CUPRAC and metal chelating assaysa.
From: Phytochemical profiling and various biological activities of Phlomis tuberosa L.
Parts of P. tuberosa | Samples/ Standards | β-Carotene-linoleic acid assay | DPPH• assay | ABTS•+ assay | CUPRAC assay | Metal chelating assay | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
IC50 (µg/mL) | Inhibition (%) (at 200 µg/mL) | IC50 (µg/mL) | Inhibition (%) (at 200 µg/mL) | IC50 (µg/mL) | Inhibition (%) (at 200 µg/mL) | A0.50 (µg/mL) | Absorbance (at 200 µg/mL) | IC50 (µg/mL) | Inhibition (%) (at 200 µg/mL) | ||
Flowers | Hexane | > 200 | 27.81 ± 0.66 | > 200 | 17.15 ± 0.20 | > 200 | 19.81 ± 0.51 | > 200 | 0.38 ± 0.01 | > 200 | 35.54 ± 0.76 |
Methanol | 48.35 ± 0.84 | 77.20 ± 0.95 | 73.56 ± 0.51 | 72.08 ± 0.48 | 51.93 ± 0.85 | 76.85 ± 0.65 | 65.43 ± 0.27 | 0.86 ± 0.08 | 158.3 ± 0.97 | 53.44 ± 1.12 | |
Aerial parts | Hexane | > 200 | 40.12 ± 0.51 | > 200 | 24.57 ± 0.34 | > 200 | 37.57 ± 0.60 | > 200 | 0.44 ± 0.05 | > 200 | 47.80 ± 0.88 |
Methanol | 61.12 ± 0.50 | 75.39 ± 0.46 | 66.47 ± 0.28 | 74.58 ± 0.90 | 52.80 ± 0.39 | 77.04 ± 0.75 | 68.51 ± 0.28 | 0.84 ± 0.07 | 122.8 ± 1.17 | 59.10 ± 0.70 | |
Roots | Hexane | > 200 | 20.27 ± 0.18 | > 200 | 16.29 ± 0.23 | > 200 | 27.71 ± 0.34 | > 200 | 0.33 ± 0.02 | > 200 | 33.20 ± 0.72 |
Methanol | 85.47 ± 0.94 | 64.14 ± 0.69 | 123.9 ± 0.55 | 58.70 ± 0.84 | 77.37 ± 1.05 | 73.10 ± 0.46 | 95.78 ± 0.35 | 0.62 ± 0.04 | 195.1 ± 1.25 | 51.27 ± 1.08 | |
Flowers | Essential oil | 171.4 ± 1,07 | 5129 ± 0.32 | > 200 | 37.23 ± 0.36 | > 200 | 39.23 ± 0.74 | > 200 | 0.43 ± 0.08 | > 200 | 43.15 ± 0.97 |
Aerial parts | Essential oil | 194.7 ± 0,89 | 50.03 ± 0.64 | > 200 | 34.73 ± 0.58 | > 200 | 41,17 ± 0.88 | > 200 | 0.45 ± 0.12 | > 200 | 42.67 ± 0.53 |
α-Tocopherol | 2.10 ± 0.05 | 95.73 ± 0.44 | 38.20 ± 0.50 | 84.25 ± 0.36 | 34.75 ± 0.55 | 83.63 ± 0.34 | 60.45 ± 0.30 | 0.88 ± 0.03 | NT | NT | |
BHA | 1.50 ± 0.03 | 96.20 ± 0.28 | 19.50 ± 0.30 | 87.90 ± 0.44 | 12.70 ± 0.10 | 88.93 ± 0.21 | 25.40 ± 0.42 | 2.45 ± 0.07 | NT | NT | |
EDTA | NT | NT | NT | NT | NT | NT | NT | NT | 5.50 ± 0.25 | 94.40 ± 0.35 | |
