Fig. 4 | Scientific Reports

Fig. 4

From: Evaluation of DNA extraction kits for long-read shotgun metagenomics using Oxford Nanopore sequencing for rapid taxonomic and antimicrobial resistance detection

Fig. 4

Performance of taxonomy identification. a and b Heatmaps with the mean read counts (log10 scale) from the raw reads for the Zymo Mock Community and the ESKAPE Mock using Kraken2. Kits used were BS, Buccal Swab; CC, Cultured Cells; DM, QIAamp DNA Mini; and PF, QIAamp PowerFecal Pro DNA. The total number of tested samples is provided inside the brackets(x-axis) which represent the technical and biological replicates for the Zymo Mock Community and the technical replicates for the ESKAPE Mock. c and d Assembled contigs alignment to the reference genomes of the Zymo Mock Community and ESKAPE Mock. The x-axis represents the different DNA extraction kits, and the y-axis represents the number of identical bases aligned to the respective reference genomes. The horizontal green line indicates the actual genome size of the respective bacterial species.

Back to article page