Table 1 Mean DNA concentration.

From: Evaluation of DNA extraction kits for long-read shotgun metagenomics using Oxford Nanopore sequencing for rapid taxonomic and antimicrobial resistance detection

Sample type

Buccal Swab - Promega

(BS, ng/µL)

Cultured Cells - Promega

(CC, ng/µL)

QIAamp DNA Mini - Qiagen (DM, ng/µL)

QIAamp Power Fecal Pro - Qiagen (PF, ng/µL)

Zymo Mock Community (n = 12)

A260/280

45.1 ± 20.0

2.0 ± 0.1

38.6 ± 7.7

2.0 ± 0.0

12.6 ± 5.1

2.1 ± 0.1

10.1 ± 2.1

2.0 ± 0.2

ESKAPE Mock (n = 3)

A260/280

268.6 ± 96.8

2.0 ± 0.1

59.2 ± 24.0

2.0 ± 0.1

40.0 ± 31.8

1.9 ± 0.0

33.7 ± 7.9

1.8 ± 0.0

Pooled swab samples (n = 9)

A260/280

245.8 ± 89.1

1.8 ± 0.0

87.9 ± 18.1

1.9 ± 0.0

49.2 ± 13.3

1.9 ± 0.0

44.6 ± 3.3

1.9 ± 0.0

  1. The mean and standard deviations of DNA concentrations and the A260/280 ratio resulting from the four different DNA extraction kits are shown. The number of samples is indicated in closed brackets.
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