Fig. 4 | Scientific Reports

Fig. 4

From: Male and female behavioral variability and morphine response in C57BL/6J, DBA/2J, and their BXD progeny following chronic stress exposure

Fig. 4

Parental C57BL/6J and DBA/2J mice have distinct place conditioning responses to morphine after chronic stress exposure. (a) Experimental timeline illustrating the two procedures performed, i.e. CSDS or CVS, prior to performing morphine conditioned place preference (CPP). Morphine CPP was performed using an unbiased approach: one compartment was paired with saline (0.3 mL, s.c.) and the other paired with morphine (7.5 mg/kg, s.c.). Sessions were 20 min long. (b) Time spent in each compartment of the three-chamber apparatus after paired-conditioning with morphine (vs. saline) in CSDS-exposed C57BL/6J and DBA/2J male mice (n = 8) and respective percentage in the morphine-paired chamber. (c) Same as (b), in CSDS-exposed C57BL/6J and DBA/2J female mice (n = 8). (d) Time spent in each compartment of the three-chamber apparatus after paired-conditioning with morphine (vs. saline) in CVS-exposed C57BL/6J and DBA/2J male mice (n = 8) and respective percentage in the morphine-paired chamber. (e) Same as (b), in CVS-exposed C57BL/6J and DBA/2J female mice (n = 8). Bars represent mean ± SEM. Two-way ANOVA followed by post-hoc comparison to C57BL/6J mice with Bonferroni correction and non-paired t-test; see Supplementary Table S1; *P < 0.05, **P < 0.01, and ***P < 0.01.

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