Fig. 2
Pancreatic acinar, but not ductal or islet cells, exhibit positive staining with mCherry-SHA in mice. (A) Co-staining of adult (15 weeks-old) murine pancreas with mCherry-SHA, Cpa1 (an acinar cell marker) and Krt19 (a ductal cell marker). Representative photomicrographs from formalin-fixed paraffin-embedded pancreas sections are shown. Bars = 50 μm. (B) Control mCherry was used instead of mCherry-SHA. Bars = 50 μm. (C) Magnified images for triple-staining with mCherry-SHA, Cpa1 and Krt19. Two insets are further magnified in the right panels, revealing the localization of mCherry-SHA on the acinar cell membrane and in the cytoplasm. The arrow indicates a ductal lumen positively stained with Cpa1, an acinar cell-secreted enzyme transported by the ducts. Bars = 10 μm. Islet areas are indicated by solid white lines in (A) and (B). Images were obtained from a ZEISS LSM 880 confocal microscope.
