Fig. 3
The mCherry-SHA binding signal on acinar cells is competitively blocked by the addition of L-Rhamnose. (A–D) Competition of mCherry-SHA signal in the acinar cells with increasing doses of L-Rhamnose (0 to 400 mM). The arrows indicate a ductal lumen positively stained with Cpa1 and mCherry-SHA, which is also reduced by L-Rhamnose competition. This suggests that SHA-BGs are secreted into ducts. (E) Control staining using mCherry and Cpa1. Islet regions are delineated by solid white lines. Bars = 50 μm. Images were obtained from a ZEISS LSM 880 confocal microscope. (F) Quantification of red fluorescence signal from mCherry-SHA in Cpa1-positive cells, normalized to mCherry signal in Cpa1-positive cells of the adjacent slide. Each dot represents a mouse pancreas. One-way ANOVA was used to determine significance. *P < 0.05. **P < 0.005.
