Fig. 3
From: TAp73 and ΔTAp73 isoforms show cell-type specific distributions and alterations in cancer
Immunocytochemical analysis of antibody specificity. Non-transfected H1299 cells or cells transfected with plasmids containing genes coding for p53 family isoforms (indicated down the left side of the figure) were formalin fixed and embedded in paraffin. Sections were immunocytochemically stained with the four antibodies indicated across the top of the figure (TAp73-1.1, affinity purified rabbit anti-p73α, mouse monoclonal p73α-1.1, and mouse monoclonal ΔNp73-1.1). Positive staining is seen as a brown precipitate and nuclei were counterstained with haemotoxylin (blue). Non-transfected H1299 cells were used as a negative control. Please note that transfections were performed transiently, therefore not all cells contain the expression plasmid.
