Fig. 1
From: Gucy1α1 specifically marks kidney, heart, lung and liver fibroblasts
UIR and UUO induced murine CKD models exhibited progressive kidney fibrotic remodeling. (A) Schematic of the murine CKD progression model experimental timeline. (B and C) Picrosirius Red staining showing ECM accumulation over the course of UIR and UUO. (B) Representative whole kidney images (original magnification, ×4, 1.48 μm/px zoom, scale 1000 μm) and ×20 0.30 μm/px zoom (cortical – upper images, exemplifying location highlighted with black frames on the control kidney image, medullary – lower images, purple frames, scale 50 μm). (C) Quantification of total staining in kidney cortex and medulla, n = 4 per group, unpaired 2-tailed t-test, P *≤0.05, **≤0.01, ***≤0.001, ****≤0.0001 compared to the control. (D and E) Western blotting showing gradual fibrosis markers upregulation over the course of UIR and UUO. (D) Representative Pdgfrβ, αSma, Vim, Gapdh bands. (E) Western blotting quantification, Pdgfrβ, αSma, Vim signal normalized to Gapdh, n = 4 per group, ordinary one-way ANOVA, P *≤0.05, **≤0.01, ***≤0.001, ****≤0.0001 compared to the control. Data in scatter plots is presented as mean ± SD.
