Fig. 5
From: Gucy1α1 specifically marks kidney, heart, lung and liver fibroblasts
Gucy1α1 does not label glomerular populations compared to historically used kidney fibrosis markers. (A) Representative IF images showing Gucy1α1 (magenta), Pdgfrβ (yellow) and Vim (green), Nphs1 (white), DAPI, blue. Pdgfrβ and Vim are abundantly present inside the glomerulus, including colocalization with Nphs1-positive podocytes (shown with white arrows). Original magnification, × 60, maximal intensity projection from a Z-stack, 0.08 μm/px Nyquist zoom, scale 25 μm (B and C) Quantitative IF analysis of intraglomerular Gucy1α1, Pdgfrβ and Vim expression in the control and UIR/UUO treated kidneys. Gucy1α1, Pdgfrβ and Vim signals are normalized to Nphs1-expressing area volume and averaged from all the glomeruli captured in the imaging field, n = 4 animals per group. Gucy1α1 and Pdgfrβ (B) or Gucy1α1 and Vim (C) normalized averaged intraglomerular signals are compared using multiple unpaired t-test with FDR correction for multiple comparisons. P values are shown as P *≤0.05, **≤0.01, ***≤0.001, ****≤0.0001, n.s., not significant between each pair of markers at each timepoint. (B) q values for Gucy1α1 vs. Pdgfrβ comparisons: control: 0.001729; UIR Day 1: 0.001729, Day 4: 0.006212, Day 7: 0.017478, Day 14: 0.001729, Day 28: 0.000237; UUO Day 1: 0.037973, Day 4: 0.018007, Day 7: 0.004218, Day 14: 0.004218, Day 28: 0.021214. (C) q values for Gucy1α1 vs. Vim comparisons: control: 0.001093; UIR Day 1: 0.001279, Day 4: 0.000177, Day 7: 0.001093, Day 14: 0.000013, Day 28: 0.000005; UUO Day 1: 0.000059, Day 4: 0.000102, Day 7: 0.001838, Day 14: 0.000177, Day 28: 0.000327. Data in scatter plots is presented as mean ± SD.
