Fig. 6
From: Gucy1α1 specifically marks kidney, heart, lung and liver fibroblasts
Gucy1α1 marks kidney fibroblasts in the female model of murine CKD. (A and B) Picrosirius Red representative images and quantification showing the effects of prolonged UIR on the female kidney. (A) Original magnification, ×20, 0.30 μm/px zoom, scale 50 μm. (B) Quantification of total staining in kidney cortex and medulla, n = 3–5 per group, unpaired 2-tailed t-test, P *≤0.05, **≤0.01, ***≤0.001, ****≤0.0001 compared to the control. (C) qPCR showing Gucy1α1 expression changes in the female CKD model. Relative expression normalized to 18s, shown as fold change, n = 3–5 per group, ordinary one-way ANOVA, ****≤0.0001 compared to the control. (D and E) Western blotting revealing that 50 and 60 min UIR caused significant Gucy1α1 upregulation in the female CKD model. Representative bands (D) and quantification (E), Gucy1α1 signal normalized to Gapdh, n = 2–4 per group, ordinary one-way ANOVA, P *≤0.05, ***≤0.001 compared to the control. (F) IF images showing Gucy1α1 expression in the female normal and fibrotic kidneys. 50 min UIR caused remarkable stromal expansion, accompanied by fibrosis markers Gucy1α1 (magenta), Pdgfrβ (white), αSma (cyan) and Vim (green) elevation between Krt8 (yellow) positive injured epithelial tubules. Note colocalization between Gucy1α1 and Pdgfrβ (white arrows), αSma (cyan arrows) and Vim (green arrows) in both control and fibrotic conditions. DAPI, blue, original magnification, × 60, maximal intensity projection from a Z-stack, 0.14 μm/px Nyquist zoom, scale 25 μm (left side) and 0.06 μm/px Nyquist zoom, scale 10 μm (right side, highlighted with white frames). Data in scatter plots is presented as mean ± SD.
