Fig. 2

Deletion of Wwox changed sleep duration without affecting the circadian rhythm in Drosophila. (A) Relative Wwox mRNA levels in control (w¹¹¹⁸) and Wwox loss-of-function mutant (Wwox^f04545) flies. Values indicate average ± SEM of three independent experiments. Student’s t-test: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (B) Sleep profiles of w¹¹¹⁸ and Wwox^f04545 male flies under a 12-h light:12-h dark cycle (ZT0: light on, ZT12: light off). Values indicate sleep duration per 30 min bins, mean ± SEM (w¹¹¹⁸, n = 32; Wwox^f04545, n = 32). (C, F) Minutes of sleep and average bout length (ABL) of sleep in individual flies were analyzed. Values represent average ± SEM (each genotype, n = 32). Mann-Whitney U test : *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (D). Activity profiles of w¹¹¹⁸ and Wwox^f04545 male flies under a 12-h light:12-h dark cycle. Values indicate beam crossing count per 30 min bins, mean ± SEM (w¹¹¹⁸, n = 32; Wwox^f04545, n = 32). (E) Free-running period and rhythmicity (inside each bar) of given genotype of flies are shown. Values are represented as mean ± SEM (w¹¹¹⁸, n = 32; Wwox^f04545, n = 30). SEM, standard error of the mean.