Fig. 1
Wogonin inhibits proliferation of GC cells. (A) Viability of SGC-7901, BGC-823 and MKN45 cells treated with or without 30µM wogonin was analyzed by CCK8 assay. (B) CCK8 assay of SGC-7901, BGC-823 and MKN45 cells treated with wogonin at different concentrations for 48 h. (C) Colony formation assays to assess the clone formation rate of SGC-7901, BGC-823 and MKN45 cells treated with wogonin at different concentrations. (D) The effect of wogonin on DNA replication was analyzed by EdU incorporation assays. (E) Tumor formation by SGC-7901 cells in nude mice. 200µL 8 × 106 SGC-7901 cells were injected subcutaneously into each mouse. Mice were randomly divided into 2 groups and were treated with wogonin (60 mg/kg) or DMSO (as control) per day for 12 d. Tumor volume was measured every 3 d by vernier caliper. At the end mice were sacrificed and the tumor weight were measured. *p < 0.05 vs. DMSO group, **p < 0.01 vs. DMSO group, ***p < 0.001 vs. DMSO group.
