Fig. 6
Wogonin inhibits STAT3 activation in GC cells. (A) Levels of total STAT3 and p-STAT3 in SGC-7901 and BGC-823 cells treated with wogonin at different concentrations for 48 h were analyzed by western blotting. (B) Levels of total AKT and p-AKT in SGC-7901 and BGC-823 cells treated with wogonin at different concentrations for 48 h were analyzed by western blotting. (C) SGC-7901 cells were transfected with STAT3 responsive luciferase reporter. After 24 h, cells were treated with wogonin at different concentrations for another 24 h and luciferase assays were performed (***p < 0.001 vs. DMSO group). (D) Levels of STAT3 protein in nucleus or cytoplasm of SGC-7901 and BGC-823 cells treated with 60 µM wogonin for 48 h were analyzed by western blotting. (E) CCK8 assay of wogonin treated SGC-7901 cells treated with or without STAT3 inhibitor stattic (***p < 0.001 vs. DMSO group).
