Fig. 2

Four different integration methods, namely iMAT, E-flux, INIT, and mCADRE are implemented for the context specific GSM model reconstruction from Human1. (a) The number of reactions and metabolites incorporated in each situation (healthy, M1 phase and M2 phase) by different methods is displayed through spider plots. In all three plots orange represents the number of metabolites and maroon represents the number of reactions. Each number of reactions and metabolites is scaled by 1000 (for example 3,967 is plotted as 3.697 and so on) to have better visualization. Methods like iMAT and mCADRE generate significantly smaller GSM models in comparison to INIT and E-flux. (b) A bar graph displaying the number of reactions carrying fluxes for each state of alveolar macrophage shows that E-flux GSM models has the highest number of reactions carrying fluxes in all of the cases. Hence, for the purpose of this study, we used E-flux generated GSM models.