Fig. 1
From: Refining minimal engineered receptors for specific activation of on-target signaling molecules
Differential localization of MERs revealed significantly different signaling intensities. (a) The molecular architecture of the constructed MERs. The myristoylated (Myr), cytoplasmic (Cyt), and transmembrane (TM) type receptors were designed. The STAT3-binding motif (STAT3) or its tyrosine-to-phenylalanine mutant (STAT3F) were incorporated into the signaling domain of the MERs. (b) Signaling analysis. Parental Ba/F3 cells and the transductants were stimulated with no ligand (-), 50 nM AP20187 (+), or 1 ng/mL IL-3 (IL3). Blots with the phospho-specific antibodies detected activation levels of signaling molecules, whereas blots with anti-Myc tag detected expression levels of the MERs (n = 1). Blots with anti-whole signaling molecules and anti-GAPDH serve as loading controls (n = 1). Uncropped blot images are provided in Supplementary Information (Supplementary Fig. 6).
