Fig. 2
PPARγ and EGR1 transcription factors have roles in MSC anti-inflammatory signaling in Scnn1b-Tg mice. Mice were given 5 × 105 MSCs intravenously at 4 weeks of age before collection of tissue/cells for analysis at the 24 h time point. (a) Schematic of the 24 h MSC study. (b) Q-PCR of PPARγ expression in lung tissue 24 h post MSC installation, n = 7–12 mice per group. Data represents mean ± SEM, statistical analysis was conducted ANOVA with Sidak’s multiple comparison test, with * = P ≤ 0.05 and **** = P ≤ 0.0001. (c) Western blot for nuclear accumulation of PPARγ and normalized densitometry using Lamin B1 expression. (d) Q-PCR analysis of Egr1, Cxcl1, Il1β, Il6, and Spp1 in lung tissue and alveolar macrophages, n = 5–8 mice per group. Data shown are expressed as group mean ± SEM with statistical analysis performed using Mann–Whitney t-test, with * = P ≤ 0.05.
