Fig. 5 | Scientific Reports

Fig. 5

From: Bone marrow mesenchymal stem cells modulate miR-202-3p to suppress neuronal apoptosis following spinal cord injury through autophagy activation via the AMPK, MAPK, and PI3K/AKT/mTOR signaling pathway

Fig. 5

BMMSCs enhance autophagy after SCI. (A) Western blotting was employed to interrogate the involvement of p62, LC3II/I, Beclin1, VPS34, and CTSD in elucidating the nuanced intricacies governing autophagic processes. (B–F) The quantitative analysis of protein abundance was conducted for p62, LC3II/I ratio, Beclin1, VPS34, and CTSD across the experimental groups of rats. (G, J) Immunofluorescence staining was performed to investigate the localization and potential interaction between LC3 (a marker for autophagosomes) and NeuN (a neuronal marker) in the context of SCI (scale bar = 50 µm). The quantification of LC3 immunofluorescence staining is depicted alongside representative images. (H, I) Immunofluorescence staining was conducted to examine the expression and potential co-localization patterns of p62 (a selective autophagy substrate) and NeuN (a neuronal marker) in the context of SCI (scale bar = 1 mm). Quantitative analysis of p62 immunofluorescence staining is provided adjacent to representative images.

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