Fig. 7

Western blot analysis was conducted for the comparison of melanin-related protein expressions in B16F10 cells in both the presence and absence of the peptide VY-9 for 48 h at concentrations of 0.2, 0.4, 0.8, and 1.6 µM. (A) Western blotting; and (B) Relative intensity of the protein band quantified by ImageJ software, with the value normalized to match the corresponding loading control. All data are shown as mean ± SD. Post hoc analysis was conducted using Dunnett’s test. Significant differences compared to the control group are indicated as follows: *p < 0.05 and **p < 0.01. Arbutin (0.2 mM) served as the positive control.