Table 1 The IC50 values of the TYR inhibitory activity of BPPH derived from different enzymes.

From: Bee pollen peptides as potent tyrosinase inhibitors with anti-melanogenesis effects in murine b16f10 melanoma cells and zebrafish embryos

Enzyme % (w/v)

Neutrase

Flavourzyme

Alcalase

Pepsin/Pancreatin

Papain

 

L-Tyrosine (mono-phenolase activity) IC50 (µg/mL)

1.0

139.90 ± 1.74e

293.63 ± 2.05i

243.00 ± 5.50h

69.76 ± 0.35b

224.30 ± 10.10g

2.5

136.60 ± 9.18e

98.32 ± 0.19c

223.97 ± 7.37g

56.29 ± 0.44a

189.90 ± 4.19f

5.0

50.01 ± 1.15a

53.69 ± 2.84a

117.67 ± 2.16d

51.57 ± 0.98a

65.89 ± 5.92b

 

L-DOPA (di-phenolase activity) IC50 (µg/mL)

1.0

180.20 ± 4.92f

286.30 ± 10.77i

174.70 ± 8.29f

63.80 ± 159c

275.93 ± 8.62h

2.5

118.43 ± 6.10d

48.19 ± 0.68ab

158.20 ± 8.97e

56.82 ± 2.82bc

267.10 ± 4.69h

5.0

39.93 ± 0.60a

43.20 ± 5.78a

65.54 ± 8.78c

42.80 ± 1.15a

248.40 ± 3.20g

  1. Data are expressed in the form of mean ± standard error and the results are generated in triplicate. a-hThose values with different letters appearing in the same row show significant differences (P < 0.05).
  2. The IC50 values of kojic acid as a positive control were 16.50 ± 1.95 µg/mL for mono-phenolase and 118.23 ± 3.19 µg/mL for di-phenolase.