Fig. 5

Histogram of quantity distribution of differentially expressed protein (A). Volcano plot of DEPs in the test and the sham group (B, C, D). Each dot represents a protein, with red representing significantly up-regulated differentially expressed proteins, blue representing significantly down-regulated differentially expressed proteins, and gray representing non-differentially expressed proteins. Relative expression of protein in Protein-seq (E). The horizontal and vertical coordinates represent genes name and the relative expression values. Relative expression log2FC refers to the logarithmic value (base 2) for the fold change of differentially expressed genes between the test and sham group. Expression of Aldoa (F), Nme2 (G) and RacGAP1 (H) in serum was verified by ELISA. (n = 5 per group) (L means 60 kPa group, M means 90 kPa group, H means 120 kPa group, S means sham group, *p < 0.05 vs. Sham, &p < 0.05 vs. 60 kPa).