Fig. 2 | Scientific Reports

Fig. 2

From: Targeting cholangiocarcinoma cells by cold piezoelectric plasmas: in vitro efficacy and cellular mechanisms

Fig. 2

Localized thermal effects induced by CPP. (a) 3D-view of the setup for infrared camera (IRcam) observation of a culture medium exposed to a CPP device. In this scheme, the helium flow driver is absent to show plasma-liquid interaction inside a well. The bottom of the well is 2D-represented with the picture obtained for a 0.5 min-Pz-CD exposure. (b) Radial distribution of the temperature collected from infrared images, aligned on the right border of the graphs at the current plasma treatment times (0, 0.5, 1, 2, 3 and 5 min). Dot lines correspond to r = 10 mm. (c) Kinetics evolution of the thermal effect for the two sources, beginning at room temperature for 30 s, helium injection during the next 30 s; then, plasma ignition from 0 to 5 min, and finally, helium and plasma turned off for the last 9 min. Temperature variation ΔT corresponds to the difference between the current temperature and the initial one. ΔTmean (gray line), ΔTmin (blue line) and ΔTmax (red line) are respectively the mean value, minimum value and maximum value recorded on the area of the well. (d) Evaporation of the culture medium on a well immediately (0 h) and 24 h after CPP treatment. The linear regression is performed with a great determination coefficient (0 h: r2 > 0.99 – 24 h: r2 > 0.9) for each source.

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