Fig. 4
Evaluating Prognostic Model Significance by Assessing the Impact of FTH1, SOD2, and CXCL8 Knockdowns on HeLa Cell Proliferation and Migration. (A) Displaying the importance scores of the four genes identified as significant markers by the random survival forest analysis. (B) Venn diagram illustrating the overlap between high-risk genes (FTH1, SOD2, and CXCL8) and key prognostic genes (FTH1, SOD2, CXCL8, and SELL), showing the intersection which includes FTH1, SOD2, and CXCL8. (C) Immunohistochemical staining reveals that the expression levels of FTH1, SOD2 and CXCL8 are significantly higher in the cancerous tissues of patients compared to the adjacent non-cancerous tissues. (D) After individually knocking down CXCL8, FTH1, or SOD2 using siRNA, the expression level of CXCL8, FTH1, or SOD2 was detected by qRT-PCR analysis. (E) MTT assay results show that the proliferation of HeLa cells is inhibited after knocking down FTH1, SOD2 or CXCL8 respectively. **P < 0.01, ***P < 0.001. (F) Scratch assay results indicate that the migration of HeLa cells is inhibited after knocking down FTH1, SOD2 or CXCL8 respectively. ****P < 0.0001. (G) Transwell assay results demonstrate that the invasion of HeLa cells is inhibited after knocking down FTH1, SOD2 or CXCL8 respectively. ****P < 0.0001.
