Fig. 8
The absence of CXCL8 inhibits macrophage proliferation and promotes an increase in the M1/M2 ratio. (A) MTT assay results show that knocking down CXCL8 in macrophages M0 inhibited their proliferation, whereas adding extra CXCL8 promoted proliferation. (B, C) MTT assay and Ki67 immunofluorescence staining indicated decreased proliferation ability in CXCL8-deficient macrophages M0 polarized into M1 and macrophages M2. (D) WB analysis shows that the expression of the macrophage biomarker CD68 decreased in CXCL8-deficient macrophages M0; the M1 biomarker CD86 increased and the M2 biomarker CD206 decreased after polarization. (E) Flow cytometry analysis confirmed that CXCL8-deficient macrophages M0 exhibited decreased CD68 expression. Additionally, an increase in CD86 was observed after polarization into macrophages M1, while a decrease in CD206 was noted following polarization into macrophages M2.
