Fig. 9
The expression of CXCL8 in M0 macrophages influences the proliferation, migration, and invasion of co-cultured HeLa cells. (A) Schematic representation of the co-culture system used to study the effect of CXCL8 expression in macrophages M0 on HeLa cells. THP1-polarized macrophages M0 were co-cultured with HeLa cells. (B) Ki67 immunofluorescence staining indicates that the proliferation of HeLa cells is inhibited when CXCL8 was knocked down in macrophages M0, whereas the addition of exogenous CXCL8 promotes HeLa cell proliferation. (C) Scratch assay results demonstrates that the migration of HeLa cells is inhibited by the deletion of CXCL8 in macrophages M0 and enhanced by the addition of exogenous CXCL8. (D) Transwell assay results show that the invasion of HeLa cells is suppressed when CXCL8 was knocked down in macrophages M0 and promoted when exogenous CXCL8 was added.
