Fig. 1
From: [Gly14]-Humanin ameliorates high glucose-induced endothelial senescence via SIRT6
High glucose induced endothelial senescence in a concentration- and time‐dependent manner. (A) Representative images of SA-β-gal staining and (B) quantification of SA-β-gal positive HUVECs treated with normal glucose (5.5 mM d-glucose), high glucose (15 mM or 30 mM d-glucose) for 24 h,48 h, and 72 h. Scale bar = 20 μm. (C) Cell apoptosis assay of HUVECs treated with normal glucose (5.5 mM d-glucose), high glucose (15 mM or 30 mM d-glucose) observed by flow cytometry. (D) Analysis of cell apoptosis results of HUVECs. The values are expressed as the mean ± SEM. Experiments were carried out in triplicate and repeated three times. Results in B and D were analyzed with two-way ANOVA with Tukey’s multiple comparisons test.
