Fig. 1 | Scientific Reports

Fig. 1

From: Individual and sex differences in frontloading behavior and approach- avoidance conflict preference predict addiction-like ethanol seeking in rats

Fig. 1

Experimental timeline and procedures. Timeline of experimental procedures (A). All animals first underwent mixed valence cue conditioning, followed by a cue-conflict preference test in the Y-maze to assess approach-avoidance conflict bias (B). Rats were then pre-exposed to ethanol in their homecages using the Intermittent Access 2 Bottle Choice (IA2BC) paradigm. Next, rats were trained to self-administer ethanol on progressively more effortful schedules of reinforcement and shorter sessions to facilitate bingeing (FR1 2 h, FR1 30 min, FR3 30 min, FR3 15 min). Finally, animals were administered three different tests that capture addiction-like measures (ethanol-seeking with progressive ratio break point (C), extinction (D), and resistance to shock (E)). Animals were given retraining sessions on FR3 15 min schedule between each test.

Back to article page