Fig. 3 | Scientific Reports

Fig. 3

From: Generation of a genetically engineered porcine melanoma model featuring oncogenic control through conditional Cre recombination

Fig. 3

Production of transgenic piglets with TB-pTYR::Cre transgene constructs. (A) Image of surrogate F11-58’s ovary during embryo transfer. (B) Picture of pregnancy diagnosis from surrogate F11-58 on day 28 after embryo transfer. Bold arrow, embryonic sac. (C) Image of TB-pTYR::Cre piglets. P, piglet. (D) Representative images showing EGFP expression in umbilical cord cells from each TB-pTYR::Cre piglet. Scale bar, 300 μm. (E) Images show EGFP expression detected in hooves under ultraviolet light in each TB-pTYR::Cre piglet. Thin arrow, EGFP expression. NC, negative control. (F) Transgene identification in genomic from the umbilical cord of TB-pTYR::Cre piglets via PCR analysis. Control, normal pig fetal fibroblasts. RN18S was used as a control. (G) Survival curve of TB-pTYR::Cre pigs (n = 5 for each group). (H) Body weight of TB-pTYR::Cre pigs (n = 3 for each group). Data are presented as mean ± SEM. The data were analyzed using Student’s t-test.

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