Fig. 4 | Scientific Reports

Fig. 4

From: Generation of a genetically engineered porcine melanoma model featuring oncogenic control through conditional Cre recombination

Fig. 4

In vitro validation of the Cre-loxP system in TB-pTYR::Cre pigs. (A) PCR analysis of genomic DNA of TB-pTYR::Cre pig fibroblasts after Cre-3NLS introduction. The arrows indicate shortened PCR amplificons resulting from Cre recombination and the excision of the EGFP-pA sequence. (B) Western blot detection of BRAFV600E, phosphorylated ERK1/2, phosphorylated AKT, and p53R167H in control and Cre-3NLS-introduced TB-pTYR::Cre pig fibroblasts. Red arrows indicate p53R167H expressed from the oncogene cassettes, which are slightly larger than wildtype p53 due to the remaining 2A peptides.

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