Fig. 6

TB-pTYR::Cre pig generation via natural mating. (A) Pedigree of a cross between a founder (F0) TB-pTYR::Cre P#3 pig and four WT pigs. (B–E) Transgene identification in genomic DNA isolated from F1-1, F1-2, F1-3, and F1-4 generation using PCR analysis. NC, negative control. P, piglet. RN18S was used as a control. (F) Images of EGFP expression detected in hooved under ultraviolet light in each F1 TB-pTYR::Cre piglet. Thin arrow, EGFP expression.